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National Centre for Disease Control

Directorate General of Health Services, Ministry of Health & Family Welfare
Government of India

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Enterovirus Division

Introduction
The Enterovirus Division has WHO Accredited National Polio Laboratory and National Measles & Rubella Laboratory. It carries out Waste-water Surveillance for Polio, COVID-19 and Other Viruses. It provided diagnostic tests for Epstein Barr Virus, Parvo-B19 Virus, Mumps and Varicella-Zoster Virus.
The Laboratory is a part of
  1. Global Polio Laboratory Network, WHO
  2. Measles & Rubella Laboratory Network, WHO
The Division is also carrying out the Project “Genomic monitoring of COVID-19 through Waste Water Surveillance”
Enterovirus Division

Staff Details


Head of the Division and Officers


Dr. Simrita Singh

MBBS, MD

Additional Director, NCDC

Dr. Purva Pankaj Sarkate

MBBS, MD, DHM

Joint Director

Dr. Purbasha Bera

MBBS, MD

Joint Director

Dr. Anupam Kumar Anveshi

MBBS, MD

Assistant Director

Dr. Usha Gupta

Ph.D.

Assistant Research Officer

Other Staff in the Division

Sh. R. Vishnu Raj Pillai

B.Sc. (H)

Technician

Sh. Deepesh

M.Sc.

Technician

Sh. Bijender Kumar

12th

Technician

Sh. Pradeep Kumar

10th pass

Technician

Ms. Chhavi

B.Tech.

Technician

Mr. Naveen Sharma

B. Sc. (Life Science)

Technician

Mr. Rahul Rojha

B.Sc. (H)

Technician

Mr. Nirmal Singh Balidani

B.Sc. (PCM)

Laboratory Assistant

Sh. Harish Kumar

12th pass

Insect Collector

Smt. D. Varalakshmi

10th pass

Insect Collector

Sh. Rishabh Kumar

BMS (Finance)

Laboratory Attendant

  1. Environmental Poliovirus Surveillance (Delhi, Uttar Pradesh)
  2. Measles and Rubella Surveillance (Delhi, Haryana, Uttarakhand, Western Uttar Pradesh Parts of Rajasthan & Madhya Pradesh).
  3. Continuous Serological and Genomic Surveillance to achieve Measles Elimination goal of the Country.
  4. Continuous Serological and Genomic Surveillance to achieve Measles Elimination goal of the Country.
    • Monitoring two Sub-National Laboratories (GMC Haldwani, SMS Jaipur).
    • Molecular testing for Measles and Rubella is done to determine the circulating genotypes.
  5. Diagnostic tests for viruses such as Mumps, Varicella Zoster, Parvo Virus B 19, Epstein Barr Virus
  6. Environmental Surveillance for SARS-CoV-2 in Delhi
    • Standardization of Methodology for Environmental Surveillance of COVID-19 at NCDC
    • Estimation of SARS CoV-2 titers from wastewater from designated sites in Delhi on a weekly basis since November 2021
  7. Hospital wastewater Surveillance for SARS-CoV-2 in Delhi (Safdarjung Hospital & Hospitals attached with Lady Hardinge Medical College)

Sewage Sample Collection in the Field

sample collection

Microscopy for detection of Cytopathic Effect

Cytopathic Effect

Laboratory Infrastructure: Cell Culture

laboratory infrastructure

Molecular Testing for Measles- Rubella

rubella testing

Sewage Concentration for Environmental Surveillance

environmental_surveillance

Cytopathic Effect in Cell Lines

Normal L20B cell line without cytopathic effect

Cytopathic Effect cell

Cytopathic effect (Polio virus) in L20B cell line

polio virus

Standardization of NCDC Method for Wastewater Surveillance of COVID-19

waste water survillence


National programmes implemented through the division are as under:
The Laboratory is a part of
  1. Global Polio Laboratory Network, WHO
  2. Measles & Rubella Laboratory Network, WHO
The Division is also carrying out the Project “Genomic monitoring of COVID-19 through Waste Water Surveillance”
  1. Kaundal, Nirmal, Purva Sarkate, Charu Prakash, and Narayan Rishi. "Comparison of ELISA and dual stage real time RT-PCR to differentiate Sabin like and non-Sabin like poliovirus isolates." VirusDisease 28, no. 2 (2017): 141-145.
  2. Kaundal Nirmal, Purva Sarkate, Charu Prakash, and Narayan Rishi. "Environmental surveillance of polioviruses with special reference to L20B cell line." Virus Disease 28, no. 4 (2017): 383-389.
  3. Sarkate PP, Bera P, Kaundal N, Lovekesh, Sood S, Singh SK, Prakash C. Sewage Analysis as a Tool for Environmental Surveillance of SARS-CoV-2: Experience from Delhi, India. J Commun Dis. 2021; 53(2): 1-13.
  4. Duvesh R, Lovekesh, Sarkate P, Bera P, Singh S, Sharma S, et al. Designing effective SARS-CoV-2 wastewater surveillance system: lessons learnt from sustained monitoring in Delhi, India. BMC Infectious Diseases [Internet]. 2026;26(1). Available from: https://link.springer.com/content/pdf/10.1186/s12879-026-13330-6.pdf
  1. Cell Culture Laboratory
  2. Virus Isolation Laboratory
  3. PCR Laboratory
  4. Environmental Surveillance Laboratory
  5. Immunodiagnostics Laboratory