Viral Hepatitis & Biotechnology Division
Details of National Programmes
1. National Programmes implemented through division are as under
National Programme Name:- National Program for surveillance of Viral Hepatitis (NPSVH)
Email ID of Programme:- nvhsp.ncdc@gmail.com
Officer In Charge: Dr. Sandhya Kabra
Programme details:
2. Please upload any relevant photograph of Division
3. Mandate of the Division: Viral Hepatitis Division:- Viral Hepatitis Laboratory at NCDC
as a centre of excellence is a NABL accredited laboratory for testing of viral markers and was upgraded as a
division in 2018 when the National Viral Hepatitis Control Program was launched. Laboratory is also
coordinating with the National Program for Surveillance of Viral Hepatitis to estimate burden of viral
hepatitis in India and contribute in policy making for prevention strategies. The objective of the
laboratory are detection and investigation outbreaks, screening of surveillance sample received from various
states.
Mandate of the Division Biotechnology Division:- The division provides molecular diagnostic
services, molecular epidemiology, specialized training and applied research on various important
epidemic-prone diseases of public health importance to achieve the following important objectives:-
- Molecular Diagnostic support for confirmation of microbial pathogen.
- Identifying new, emerging and re-emerging pathogens.
- Genotyping and Sub-typing of strains.
- Whole Genome Sequencing using NGS platform.
Staff Details
Head of the Division and Officers
HOD details not available.
Other Staff in the Division
No staff found.
Research Projects
India Epidemic Intelligence Services (EIS) “Study on seroconversion of hepatitis C and its risk factors in
chronic kidney disease patients undergoing maintenance hemodialysis in tertiary care hospitals of Delhi, 2023”
under the supervision of Dr Sandhya Kabra, Deputy Commissioner National Viral Hepatitis Control Program &
Addl. Director, National Centre Disease Control, New Delhi.
Research projects and Publication (With website link) Biotech Division:-
Publications
-
1
Pathak AK, Mishra GP, Uppili B, Walia S, Fatihi S, Abbas T, Banu S, Ghosh A, Kanampallilwar A, Jha A,
Fatma S. Spatio-temporal dynamics of intra-host variability in SARS-CoV-2 genomes. Nucleic acids research.
2022 Feb 22;50(3):1551-61.
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2
Dhar MS, Marwal R, Vs R, Ponnusamy K, Jolly B, Bhoyar RC, Sardana V, Naushin S, Rophina M, Mellan TA,
Mishra S. Genomic characterization and epidemiology of an emerging SARS-CoV-2 variant in Delhi, India.
Science. 2021 Nov 19;374(6570):995-9.
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3
Mlcochova P, Kemp SA, Dhar MS, Papa G, Meng B, Ferreira IA, Datir R, Collier DA, Albecka A, Singh S,
Pandey R. SARS-CoV-2 B.1.617.2 Delta variant replication and immune evasion. Nature. 2021
Nov;599(7883):114-9.
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4
Dhar MS, Asokachandran V, Uppili B, Tyagi N, Sharma P, Tiwari S, Srinivasan RV, Marwal R, Kanakan A, Khan
AM, Pandey R. Reinfection or reactivation: Genome-based two distinct SNP profile of SARS-CoV2 repositivity
in an Indian case. Journal of Medical Virology. 2021 Jul;93(7):4152.
Branches Under Division:
NIL
Biotechnology Division
Details of Laboratories/Units:
Hepatitis Lab/Division – 1 Research Assistant, 1 Lab Technology, 2 Lab Assistant, 1 Lab Attendant
Details of Biotechnology Lab/Division:
1 Research Assistant, 1 Lab Technology, 1 Lab Assistant, 2 Insect Collector
Laboratories under the Division:
Viral Hepatitis Lab and Biotech Lab
Viral Hepatitis Laboratory
Biotechnology Laboratory
Officer in Charge:
Dr. Sandhya Kabra and Dr. Partha Rakshit
Lab/Unit details: Details attached with S. No.10
Tests carried out at Biotechnology Division
| S.No |
Test Offered |
Pathogen |
Method |
TAT |
| 1 |
Whole Genome Sequencing / NGS Panel |
SARS-CoV-2 |
Next Generation Sequencing |
7–10 working days |
| Respiratory Viruses |
Next-Generation Sequencing Respiratory Viral Panel |
7–14 working days |
| Viral Pathogens |
Next-Generation Sequencing Viral Pathogen Panel |
7–14 working days |
| 2 |
Targeted Sequencing |
HBV, HCV, Dengue etc. |
Sanger Sequencing |
7–14 working days |
| 3 |
RT-PCR |
SARS-CoV-2 |
Chip based RT-PCR |
2 working days |
| Automated RT-PCR |
2 working days |
* Referral services through IDSP and other Apex laboratories of the Institutions and for surveillance purpose only. (Sample requisition form available on request basis)
** TAT may differ depending upon pathogen and batch size.
Specimen Referral Form for COVID-19 (SARS-CoV2) available at https://ncdc.mohfw.gov.in/showfile.php?lid=527
Respiratory Virus detected through Respiratory Viral Panel
Respiratory Virus Panel
- Human coronavirus 229E
- Human coronavirus NL63
- Human coronavirus OC43
- Human coronavirus HKU1
- Human parainfluenza virus 1
- Human parainfluenza virus 2
- Human parainfluenza virus 3
- Human parainfluenza virus 4a
- Influenza A virus (A/Puerto Rico/8/1934(H1N1))
- Influenza A virus (A/ Korea/426/1968(H2N2))
- Influenza A virus (A/New York/392/2004(H3N2))
- Influenza A virus (A/goose/ Guangdong/1/1996(H5N1))
- Influenza A virus (A/Zhejiang/DTIDZJU01/2013(H7N9))
- Influenza A virus (A/Hong Kong/1073/99(H9N2))
- Influenza A virus (A/ Texas/50/2012(H3N2))
- Influenza A virus (A/ Michigan/45/2015(H1N1))
- Influenza B virus (B/Lee/1940)
- Influenza B virus (B/ Wisconsin/01/2010)
- Influenza B virus (B/ Brisbane/60/2008)
- Influenza B virus (B/ Colorado/06/2017)
- Influenza B virus (B/ Washington/02/2019)
- SARS-CoV-2
- Human metapneumovirus (CAN97-83)
- Human adenovirus B1
- Human adenovirus C2
- Human adenovirus E4
- Respiratory syncytial virus (type A)
- Human Respiratory syncytial virus 9320 (type B)
- Human bocavirus 1 (Primate bocaparvovirus 1 isolate st2)
- Human bocavirus 2c PK isolate PK-5510
- Human bocavirus 3
- Human bocavirus 4 NI strain HBoV4- NI-385
- KI polyomavirus Stockholm 60
- WU Polyomavirus
- Human parechovirus type 1 PicoBank/HPeV1/a
- Human parechovirus 6
- Human rhinovirus A89
- Human rhinovirus C (strain 024)
- Human rhinovirus B14
- Human coronavirus HKU1
- Human enterovirus C104 strain: AK11
- Human enterovirus C109 isolate NICA08-4327
- Human control genes
Viral Pathogen Panel (NGS - 66 Pathogens)
Tests at Viral Hepatitis Laboratory
| Section |
Tests offered |
Section |
| Viral Hepatitis Lab |
HBsAg (Rapid) |
24 hrs |
| HBsAg (RAPID) |
24 hrs. |
| Anti HCV (RAPID) |
24 hrs. |
| HBsAg ELISA |
7 days |
| Anti HCV ELISA |
7 days |
| Anti HBs ELISA |
7 days |
| Anti HAV – IgM ELISA |
7 days |
| Anti HEV-IgM ELISA |
7 days |
| HBc-IgM ELISA |
7 days |
| Anti HBc ELISA |
7 days |
| HBeAg ELISA |
7 days |
| HBeAb ELISA |
7 days |
| Anti HAV IgM (CLIA) |
24 - 48 hrs |
| HBsAg (CLIA) |
24 - 48 hrs |
| Anti HCV (CLIA) |
24 - 48 hrs |
| HBV (VIRAL LOAD), COBAS 6800 |
10 days |
| HCV (VIRAL LOAD), COBAS 6800 |
10 days |
