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Centre for Arboviral and Zoonotic Diseases

Mandate of the Division:

Centre for Arboviral And Zoonotic Diseases comprises of 9 National Reference Laboratory dealing with Zoonotic diseases of public health importance including outbreak prone and emerging infectious diseases. The role of the division is primarily to provide laboratory evidence by conducting special and reference level tests which are not available at most institutes or medical colleges in India. Currently the work is being carried out on the following Zoonotic diseases: Rabies, Kala-azar, Arboviral infections (Dengue, JE, Chikungunya, Zika virus & CCHF), Toxoplasmosis, Brucellosis, Leptospirosis, Rickettsiosis, Hydatidosis, Neurocysticercosis, Plague and Anthrax etc. CAZD is also involved in Plague surveillance and preparedness assisted with Plague surveillance unit, NCDC Bengaluru Branch.

Relevant Photographs of the Division

Training on PPE, Spill Management & Triple Layer Packing, 2019

PHOTOGRAPH OF CAZD (FORMERLY ZOONOSIS DIVISION) OFFICIERS AND OFFICIALS, NCDC, DELHI, 2017

LABORATORY EXPERT GROUP MEETING FOR PROGRAMMES UNDER, DIVISION OF ZOONOTIC DISEASE PROGRAMMES, 2021

Hands-on Training on Diagnostic Rickettsiology, 2020

BIOSAFTETY AND BIOSECURITY WORKSHOP UNDER HISICON-2021, ORGANISED BY CAZD

OFFICIER AND OFFICIALS OF ZOONOSIS DIVISION FORMED IN 1963 (PRESENTLY KNOWN AS CENTRE FOR ARBOVIRAL AND ZOONOTIC DISEASES

Publications

Research Papers

Jayaprakasam M, Chatterjee N, Chanda MM, Shahabuddin SM, Singhai M, Tiwari S, Panda S. Human anthrax in India in recent times: A systematic review & risk mapping. One Health. Volume 16, June 2023, 100564. https://doi.org/10.1016/j.onehlt.2023.100564

Goyal R, Singhai M, Mahmood T, Saxena V. Association between the physical activity and metabolic syndrome in residents of a foot-hill area in India. Diabetes Metab Syndr. 2022 Mar 23;16(4):102471. doi: 10.1016/j.dsx.2022.102471

Agrawal SJ, Goyal R, Singhai M. Breakthrough Infections and Omicron Variant: Dealing with the Dilemma. Indian J Med Biochem 2021; 25 (3):0-0.

Singhai, M.; Sood, V.; Singh, G.; Siddiqui, C.; Nale, T.; Jha, P.; Yadav, P.; Jaiswal, R.; Bala, M.; Singh, S.K.; Tiwari, S. Rabies Outbreak in the Urban Area of Delhi: An Investigation Report and One Health Perspective for Outbreak Management. Infect. Dis. Rep. 2022, 14, 1033-1040.

Singhai M, Sood V, Yadav P, Kumar KK, Jaiswal R, Madabhushi S, Dhull P, Bala M, Singh SK, Tiwari S. Intravitam Diagnosis of Rabies in Patients with Acute Encephalitis: A Study of Two Cases. Infect Dis Rep. 2022 Nov 29;14(6):967-970. doi: 10.3390/idr14060095. PMID: 36547241; PMCID: PMC9778139.

Singhai, M., Shah, Y. D., Gupta, N., Bala, M., Kulsange, S., Kataria, J., & Singh, S. K. (2021). Chronicle down memory lane: India’s sixty years of plague experience. Indian Journal of Medical Microbiology.

Singhai M, Jaiswal R, Siddiqui C, Tiwari S, Gupta N, Bala M, Singh SK. Rabies can be a disease of puppyhood. J Family Med Prim Care. 2022 Jun;11(6):3339-3341. doi: 10.4103/jfmpc.jfmpc_1605_21. Epub 2022 Jun 30. PMID: 36119256; PMCID: PMC9480633.

Arora, R., Goel, R., Singhai, M., Gupta, N., & Saxena, S. (2021). Rabies Antigen Detection in Postmortem Cornea. Cornea, 40(5), e10-e11.

Singhai, M., Dhariwal, A. C., Gupta, N., Goswami, S., & Singh, S. K. (2020). An Observational Study of JE Cases Detected in National Centre for Disease Control from January 2017 to December 2019. Journal of Communicable Diseases (E-ISSN: 2581-351X & P-ISSN: 0019-5138), 52(4), 12-16.

Gupta, N., Singhai, M., Garg, S., Shah, D., Sood, V., & Singh, S. K. (2020). The missing pieces in the jigsaw and need for cohesive research amidst coronavirus infectious disease 2019 global response. medical journal armed forces india, 76(2), 132-135.

Jaiswal¹, R., Chhabra, M., Singh, P., Gupta, N., Singhai, M., Dhariwal, A. C., & Tripathi, P. (2018). Molecular Epidemiology and Sequence Analysis of Rabies Virus Isolates from North and North East India. Journal of Communicable Diseases, 50(4), 4

Singh, G., Chhabra, M., Singh, P., Gupta, N. K., Singhai, M., Dhariwal, A. C., & Ram, S. (2018). Molecular Study of Glycoprotein (G) Gene Region of Rabies Virus from Spotted Deer, Delhi, India. Journal of Communicable Diseases, 50(3), 3.

Singh, G., Jaiswal, R., Chhabra, M., Sood, Y., Gupta, N., Singhai, M., Tiwari, S., Dhariwal, A.C., Sharma, R. and Ram10, S. (2017). Evaluation of Direct Rapid Immunohistochemistry Test (DRIT) for Postmortem Diagnosis of Rabies. Journal of Communicable Diseases, 49(3), 3

Rawat, V., Kumar, A., Kumar, M., Singhai, M., Rawat, C. M. S., & Jha, S. K. (2016). Viral Hepatitis A and E Outbreaks in Kumaon Region of Uttarakhand. National Academy Science Letters, 39(1), 17-19.

Singhai, M., Rawat, V., Singh, P., & Goyal, R. (2016). Fatal case report of concomitant hepatitis E and Salmonella paratyphi A infection in a sub-Himalayan patient. Annals of Tropical Medicine & Public Health, 9(1)

Malik, Y. P. S., Kumar, N., Rawat, V., Sharma, K., Kumar, A., Singhai, M., & Kumar, M. (2015). Detection and distribution pattern of prevalent genotypes of hepatitis-C virus among chronic hepatitis patients from Kumaon region of Uttarakhand, India. Indian journal of medical microbiology, 33, 161.

Mittal, V., Chhabra, M., & Venkatesh, S. (2015). Ebola virus-an Indian perspective. The Indian Journal of Pediatrics, 82(3), 207-209

Sharma, P., Mittal, V., Chhabra, M., Singh, P., Bhattacharya, D., & Venkatesh, S. (2014). Dominance shift of DENV-1 towards reemergence and co-dominant circulation of DENV-2 & DENV-3 during post-monsoon period of 2012 in Delhi, India. Journal of Virology and Retrovirology, 1(1), 104.

Shrivastava, A., et al (2015). Outbreaks of unexplained neurologic illness—Muzaffarpur, India, 2013–2014. MMWR. Morbidity and mortality weekly report, 64(3), 49.

Sharma, P., Mittal, V., Chhabra, M., Kumari, R., Singh, P., & Venkatesh, S. (2016). Molecular epidemiology and evolutionary analysis of dengue virus type 2, circulating in Delhi, India. Virusdisease, 27(4), 400-404.

Singh, P., Chhabra, M., Sharma, P., Jaiswal, R., Singh, G., Mittal, V., Rai, A. and Venkatesh, S. (2016). Molecular epidemiology of Crimean-Congo haemorrhagic fever virus in India. Epidemiology & Infection, 144(16), 3422-3425.

Singh, P., Sharma, P., Kumar, S., Chhabra, M., Rizvi, M.A., Mittal, V., Bhattacharya, D., Venkatesh, S. and Rai, A. (2016). Continued persistence of ECSA genotype with replacement of K211E in E1 gene of Chikungunya virus in Delhi from 2010 to 2014. Asian Pacific Journal of Tropical Disease, 6(7), 564-566.

Thakur, R., Kumar, Y., Singh, V., Gupta, N., Vaish, V. B., & Gupta, S. (2016). Serogroup distribution, antibiogram patterns & prevalence of ESBL production in Escherichia coli. The Indian journal of medical research, 143(4), 521.

Kumar, Y., Gupta, N., Vaish, V. B., & Gupta, S. (2016). Distribution trends & antibiogram pattern of Salmonella enterica serovar Newport in India. The Indian journal of medical research, 144(1), 82.

Operational Research

To study the epidemiological profile of Kala-azar patients in Delhi
Serological studies in Toxoplasmosis in different Delhi Hospitals.
Comparative analysis of various serological tests in diagnosis of Toxoplasmosis.
Surveillance of Plague in different parts of the country.
Molecular characterisation of strains of Leishmania.
Standardization of appropriate diagnostic methods for sero-diagnosis and sero-epidemiology of leptospirosis.
Surveillance of arboviral infections in man and animals.
Study of prevalence of Rabies in peridomestic and wild rodents.
Standardization of Rapid Fluorescent Focus Inhibition Test (RFFIT) for rabies antibody titer.
Serological studies in clinically suspected cases of hydatid disease.
Sero-epidemiological studies for rickettsial diseases (scrub typhus & Indian tick typhus) in patient with pyrexia of unknown origin.

Sr. No	Lab/Unit name:
1.	Plague/Anthrax Laboratory
2.	Leishmania/Toxoplasma/ Cysticercosis/ Hydatid Laboratory
3.	Arbovirus Laboratory (Dengue/Chikungunya/Japanese Encephalitis)
4.	Leptospira/Brucella Laboratory
5.	Rickettsia Laboratory /Borrellia Laboratory
6.	Rabies Laboratory
7.	Molecular Laboratory
8.	Tissue Culture Laboratory
9.	Quality control and Quality assurance
10.	Central Plague Surveillance Unit

A) ROUTINE REFERRAL DIAGNOSTIC SERVICES

S.no	Disease	Name of the test	Turn Around Time *	Sample referral form(link)
1.	Leishmaniasis (Kala-azar)	Bone marrow/liver/spleen/LN aspirate Staining for LD bodies	1 day	Link for Leishmaniasis
		Culture – Schneider medium	10-14 days
		Rk 39 RDT	1 day
		IFAt	1-4 days
		PCR for Leishmania donovani	2-4 days
2.	Toxoplasmosis	Bone marrow/liver/spleen/LN aspirate Staining for LD bodies	1 day	Link for Toxoplasma
		Culture – Schneider medium	10-14 days
		Rk 39 RDT	1 day
		IFAt	1-4 days
		PCR for Leishmania donovani	2-4 days
3.	Cysticercosis	Bone marrow/liver/spleen/LN aspirate Staining for LD bodies	1 day	Link for Cysticercosis
		Culture – Schneider medium	10-14 days
		Rk 39 RDT	1 day
		IFAt	1-4 days
		PCR for Leishmania donovani	2-4 days
4.	Echinococcus	Ig G ELISA	1 day	Link of Echinococcus
		Ig M ELISA for Dengue	1-4 days
		NS1 ELISA for Dengue	1-4 days
		NS1 RDT for Dengue	1 day
5.	Dengue	Ig M RDT for Dengue	1 day	Link for Dengue
		Ig G RDT for Dengue	1 day
		PCR /QPCR for Dengue	1 day
		PCR /QPCR for Dengue Type specific	2-4 days
6.	Chikungunya	Ig M ELISA for Chikungunya	1 day	Link of Chikungunya
		Ig M RDT for Chikungunya	1 day
		Ig G RDT for Chikungunya	1 day
		PCR /QPCR for Chikungunya	2-4 days
7.	Japanese Encephalitis	Ig M ELISA for JE	1-4 days	Link for JE
7.	Japanese Encephalitis	QPCR	2-4 days	Link for JE
8.	Rabies virus	IgG ELISA Rabies antibody detection in Human serum and plasma	1-4 days	Link for antibody titre (Human)
		Ig G ELISA Rabies antibody detection in animal (dogs/cats) serum and plasma	1-4 days	Link for antibody titre (Animal)
		RDT Antigen Detection in animal brain tissue (Canine/bovine/raccoon dog)	1 day	Link for Proforma for a case of Hydrophobia (Human)
		FAT for postmortem human/animal samples	1 day	Link for Advisory and Proforma for Postmortem Sample sent for Medicolegal Purpose for Confirmation of Rabies
		RT-PCR for human/animal samples	2-4 days	Link for Epidemiology of cases of rabies in dogs & other animals
9.	Brucellosis	Ig M ELISA for Brucella	1-4 days	Link for Brucella
		Ig G ELISA for Brucella	1-4 days
		B. abortus tube agglutination test	1-4 days
		B. melitensis tube agglutination test	1-4 days
10.	Leptospirosis	Ig M for Leptospirosis	1-4 days	Link of Leptospira
10.	Leptospirosis	Ig G for Leptospirosis	1-4 days	Link of Leptospira
11.	Rickettsial diseases	Weil felix test-Ox2	1-4 days	Link for Rickettsial Diseases (Scrub Typhus)
		Weil felix test-Ox19	1-4 days
		Weil felix test-OxK	1-4 days
		Ig M ELISA for Scrub typhus	1-4 days
		Ig G ELISA for Scrub Typhus	1-4 days
12.	Lyme	ELISA	1-4 days	Link for lyme
12.	Lyme	QPCR	2-4 days	Link for lyme

Notes:

*For Samples received on working days (Monday-Wednesday) 10 am to 5 pm; turnaround time is tentative subject to availability of optimum batch size for complete utilization of kits Kindly contact concerned official/officer for information required regarding of case Investigation form, sample type, time, collection and transport. kit availability etc. before sending the samples Dr. Monil Singhai, JD /Dr. Alka Singh, MO, CAZD Contact No. 011-23913811, 011-20832482 Email id: nicdzoonosis@yahoo.com

S.no	Name of the Disease	Name of the test	Turn Around Time
1.	CCHF	Ig M ELISA for CCHF	1 day
		Ig G ELISA for CCHF	1 day
		QPCR	2-4 days
2.	Dengue	Ig G ELISA for Dengue	1 day
3.	Dengue	Ig G ELISA for Dengue	1 day
4.	Zika Virus Disease Link for Zika proforma	Ig M ELISA for Zika	1 day
		Ig G ELISA for Zika	1 day
		RT-PCR /QPCR	2-4 days
5.	Brucellosis	Staining /Culture and Sensitivity	1 day
6.	Leptospirosis	RDT for Leptospirosis	1 day
		Microscopy: Dark field microscopy /Silver staining	1-4 days
		Culture on EMJH	7-10 days
		PCR for Leptospirosis	2-4 days
7.	Rickettsial Diseases	IFAT for Scrub Typhus	1-4 days
		IgM for Spotted Fever	1-4 days
		IgG for Spotted Fever	1-4 days
		IgM for Typhus Group	2-4 days
		IgG for Typhus Group	1-4 days
		PCR for Scrub Typhus	2-4 days
8.	Hantaan Virus	Ig M ELISA for Hantaan virus	1 day
		Ig G ELISA for Hantaan virus	1 day
		QPCR	2-4 days
9.	Chandipura Virus	QPCR	2-4 days
10.	KFD	QPCR	2-4 days
11.	Ebolavirus Disease (Zaire)	Ebolavirus Antigen ELISA	1 day
11.	Ebolavirus Disease (Zaire)	QPCR	2-4 days
12.	Nipah Virus Disease	Ig M ELISA for Nipah	1 day
		Ig G ELISA for Nipah	1 day
		Nipah Antigen ELISA	1 day
		QPCR	2-4 days
13.	Monkey pox virus	QPCR	2-4 days
14.	Ortho pox virus	QPCR	2-4 days
15.	West nile virus	QPCR	2-4 days
16.	West nile virus	QPCR	2-4 days
17.	Plague	Microscopy: Gram staining, Wayson staining	1 day
		Culture	4-7 days
		PCR	2-4 days
18.	Anthrax	Microscopy: Gram staining, Wayson staining	1 day
		Culture	4-7 days
		PCR	2-4 days

Contact Us

Full Mailing Address:

Centre for Arboviral and Zoonotic Diseases (CAZD),
National Centre for Disease Control,
Ministry of Health and Family Welfare

www.ncdc.gov.in

011-20832481

N/A

Staff Details

Head of the Division and Officers

Dr. Naveen Kumar Gupta

MD Medical Microbiology, Fellowship Infectious Diseases, MBA, Advanced certification in Data Science, Artificial Intelligence

Additional Director & Head of the Department

cazdncdc@gmail.com

Dr. Aarti Tewari

MBBS, MD

Joint Director & Officer-In-Charge

cazdncdc@gmail.com

Dr. Shubha Garg

MBBS, MD

Joint Director

cazdncdc@gmail.com

Ms. Yosman

M.Sc

Assistant Research Officer

N/A

Ms. Niti Akoj

M.Sc

Assistant Research Officer

N/A

Dr. Rekha Jaiswal

M.Sc

Assistant Research Officer

N/A

Other Staff in the Division

Mr. Anbarasan

B.Sc

N/A

Ms Pooja Sharma

B.Sc

Technician

N/A

Mr. Sunil

N/A

Animal Attendant

N/A

Sh. Girrraj Singh

M.Sc., M. Phil PGD.MPH PGD One Health

Research Assistant

N/A

Ms. Sharda Singh

M.Sc, B. Ed

Research Assistant

N/A

Sh. Chandan Singh

B.Sc

Research Assistant

N/A

Sh. K. S. Pandey

M.Sc

Research Assistant

N/A

Sh. Vinay Singh

M.Sc

Research Assistant

N/A

Ms. Jyoti

M.Sc

Research Assistant

N/A

Ms. Preeti Khatri

M.Sc

Technician

N/A

Ms. Neha Aggarwal

M.Sc

Technician

N/A

Ms. Priyanka Yadav

M.Sc

Technician

N/A

Sh. Man Mohan Singh Mehra

N/A

Technician

N/A

Sh. Sumit Shukla

B.Sc

Technician

N/A

Ms. Saraswati Paeik

N/A

Technician

N/A

Sh. Balraj Singh

N/A

Technician

N/A

Sh. Vinod Kumar

N/A

Laboratory Assistant

N/A

Sh. Deepak Kumar

N/A

Laboratory Assistant

N/A

Sh. Suresh Kumar

N/A

Laboratory Assistant

N/A

Sh. Rakesh

N/A

Laboratory Assistant

N/A

Sh. Madan Lal

N/A

Insect Collector

N/A

Sh. Devender Singh

N/A

Insect Collector

N/A

Sh. Samar Nath

10th

Insect Collector

N/A

Ms. Rani

7th Pass

Lab Attendant

N/A

Sh. Naveen Kumar

10th

Laboratory Attendant

N/A

Ms. Maya

N/A

Animal Attendant

N/A

Sh. Man Singh Meena

8th Pass

MTS

N/A

Ms. Monica

10th

MTS

N/A

National Centre for Disease Control

Centre for Arboviral and Zoonotic Diseases

Mandate of the Division:

Relevant Photographs of the Division

Training on PPE, Spill Management & Triple Layer Packing, 2019

PHOTOGRAPH OF CAZD (FORMERLY ZOONOSIS DIVISION) OFFICIERS AND OFFICIALS, NCDC, DELHI, 2017

LABORATORY EXPERT GROUP MEETING FOR PROGRAMMES UNDER, DIVISION OF ZOONOTIC DISEASE PROGRAMMES, 2021

Hands-on Training on Diagnostic Rickettsiology, 2020

BIOSAFTETY AND BIOSECURITY WORKSHOP UNDER HISICON-2021, ORGANISED BY CAZD

OFFICIER AND OFFICIALS OF ZOONOSIS DIVISION FORMED IN 1963 (PRESENTLY KNOWN AS CENTRE FOR ARBOVIRAL AND ZOONOTIC DISEASES

Publications

Research Papers

Operational Research

Technical Guidelines

Technical Reports / Communicable Disease Alerts

Manuals

A) ROUTINE REFERRAL DIAGNOSTIC SERVICES

Notes:

Contact Us

Full Mailing Address:

Centre for Arboviral and Zoonotic Diseases (CAZD), National Centre for Disease Control, Ministry of Health and Family Welfare

www.ncdc.gov.in

011-20832481

N/A

Staff Details

Head of the Division and Officers

Dr. Naveen Kumar Gupta

Dr. Aarti Tewari

Dr. Shubha Garg

Ms. Yosman

Ms. Niti Akoj

Dr. Rekha Jaiswal

Other Staff in the Division

Mr. Anbarasan

Ms Pooja Sharma

Mr. Sunil

Sh. Girrraj Singh

Ms. Sharda Singh

Sh. Chandan Singh

Sh. K. S. Pandey

Sh. Vinay Singh

Ms. Jyoti

Ms. Preeti Khatri

Ms. Neha Aggarwal

Ms. Priyanka Yadav

Sh. Man Mohan Singh Mehra

Sh. Sumit Shukla

Ms. Saraswati Paeik

Sh. Balraj Singh

Sh. Vinod Kumar

Sh. Deepak Kumar

Sh. Suresh Kumar

Sh. Rakesh

Sh. Madan Lal

Sh. Devender Singh

Sh. Samar Nath

Ms. Rani

Sh. Naveen Kumar

Ms. Maya

Sh. Man Singh Meena

Ms. Monica

Centre for Arboviral and Zoonotic Diseases (CAZD),
National Centre for Disease Control,
Ministry of Health and Family Welfare