Viral Hepatitis & Biotechnology Division
Details of National Programmes
1. National Programmes implemented through division are as under
National Programme Name:- National Program for surveillance of Viral Hepatitis (NPSVH)
Email ID of Programme:- nvhsp.ncdc@gmail.com
Officer In Charge: Dr. Sandhya Kabra
*Programme details:
2. Please upload any relevant photograph of Division
3. Mandate of the Division: Viral Hepatitis Division:- Viral Hepatitis Laboratory at NCDC as a centre of excellence is a NABL accredited laboratory for testing of viral markers and was upgraded as a division in 2018 when the National Viral Hepatitis Control Program was launched. Laboratory is also coordinating with the National Program for Surveillance of Viral Hepatitis to estimate burden of viral hepatitis in India and contribute in policy making for prevention strategies. The objective of the laboratory are detection and investigation outbreaks, screening of surveillance sample received from various states.
Mandate of the Division Biotechnology Division- The division provides molecular diagnostic services, molecular epidemiology, specialized training and applied research on various important epidemic-prone diseases of public health importance to achieve the following important objectives:-
- Molecular Diagnostic support for confirmation of microbial pathogen.
- Identifying new, emerging and re-emerging pathogens.
- Genotyping and Sub-typing of strains.
- Whole Genome Sequencing using NGS platform.
Staff Details
Dr. Sandhya Kabra
MBBS, MD
Additional Director, NCDC
Other Officers in the Division
Dr. Partha Rakshit
MBBS, MD
Joint Director
Dr. Preeti Madan
MBBS, MD
Joint Director
Dr. Hemlata Lall
MBBS, MD
Deputy Director
Dr. Hema Gogia
MBBS, MD
Assistant Director
Other Staff in the Division
Mrs. Priyanka Singh
Ph.D.
RA, Biotechnology Division
Mrs. Uma Sharma
Ph.D.
Technician Biotechnology Division
Mr. Ramesh Sharma
Graduate (B.Sc.)
RA, Viral Hepatitis
Mr. Sattender Kumar
Post Graduate (M.Sc.)
Lab Technician, Viral Hepatitis
Mr. Kaptan
12th
IC, Biotechnology Division
Mr. Namo Narayan Meena
12th
IC, Biotechnology Division
Mr. Rajeev Kumar Sharma
12th
Laboratory Assistant, Viral Hepatitis
Mrs. Swati Kumari
M.Sc Biotechnology
Lab. Assistant, Biotechnology Division
Mr. Yogesh Kumar
11th
Lab Attendant Viral Hepatitis
Mr. Joginder Singh
10th
Lab. Assistant, Viral Hepatitis
Research Projects
India Epidemic Intelligence Services (EIS) “Study on seroconversion of hepatitis C and its risk factors in chronic kidney disease patients undergoing maintenance hemodialysis in tertiary care hospitals of Delhi, 2023” under the supervision of Dr Sandhya Kabra, Deputy Commissioner National Viral Hepatitis Control Program & Addl. Director, National Centre Disease Control, New Delhi.
Research projects and Publication (With website link) Biotech Division:-
Publications
- 1 Pathak AK, Mishra GP, Uppili B, Walia S, Fatihi S, Abbas T, Banu S, Ghosh A, Kanampalliwar A, Jha A, Fatma S. Spatio-temporal dynamics of intra-host variability in SARS-CoV-2 genomes. Nucleic acids research. 2022 Feb 22;50(3):1551-61.
- 2 Dhar MS, Marwal R, Vs R, Ponnusamy K, Jolly B, Bhoyar RC, Sardana V, Naushin S, Rophina M, Mellan TA, Mishra S. Genomic characterization and epidemiology of an emerging SARS-CoV-2 variant in Delhi, India. Science. 2021 Nov 19;374(6570):995-9.
- 3 Mlcochova P, Kemp SA, Dhar MS, Papa G, Meng B, Ferreira IA, Datir R, Collier DA, Albecka A, Singh S, Pandey R. SARS-CoV-2 B. 1.617. 2 Delta variant replication and immune evasion. Nature. 2021 Nov;599(7883):114-9.
- 4 Dhar MS, Asokachandran V, Uppili B, Tyagi N, Sharma P, Tiwari S, Srinivasan RV, Marwal R, Kanakan A, Khan AM, Pandey R. Reinfection or reactivation: Genome‐based two distinct SNP profile of SARS‐CoV2 repositivity in an Indian case. Journal of Medical Virology. 2021 Jul;93(7):4152.
6. Branches Under Division:
NIL
National Centre for Disease Control
Biotechnology Division
Details of Laboratories/Units: Hepatitis Lab/Division- 1Research Assistant, 1Lab Technology, 2Lab. Assistant, 1Lab.Attendent
Details of Biotechnology Lab/Division- 1Research Assistant, 1Lab Technology, 1Lab. Assistant, 2Insect Collector
1. Laboratories/ units under the Division: – Viral Hepatitis Lab and Biotech Lab
Lab/Unit name: Viral Hepatitis Laboratory
Lab/Unit name: Biotechnology Laboratory
Officer in Charge of Lab/Unit:- Dr. Sandhya Kabra and Dr. Partha Rakshit
Lab/Unit details: Details attached with S. No.10
Tests carried out at Biotechnology Division:
| S.no | Test offered | Pathogen | Method | Turn Around Time (TAT) |
| 1. | Whole Genome Sequencing and Next-Generation Sequencing Panel* | SARS CoV- 2 | Next Generation Sequencing | 7-10 working days ** |
| Respiratory Viruses (List attached at annexure-1) | Next-Generation Sequencing Respiratory Viral Panel | 7-14 working days ** | ||
| Viral pathogens (List attached at annexure-2) | Next-Generation Sequencing Viral Pathogen Panel | 7-14 working days ** | ||
| 2. | Targeted Sequencing (Genotyping/subtyping) * | HBV, HCV, HAV, HEV, Dengue, Chikungunya, Rabies and other pathogens. | Sanger Sequencing | 7-14 working days ** |
| 3. | Molecular Diagnosis by RT-PCR | SARS CoV-2 | Chip based RT-PCR | 2 working days |
| Automated RT-PCR | 2 working days |
* Referral services through IDSP and other Apex laboratories of the Institutions and for surveillance purpose only. (Sample requisition form available on request basis)
** TAT may differ depending upon pathogen and batch size.
Specimen Referral Form for COVID-19 (SARS-CoV2) available at
https://ncdc.mohfw.gov.in/showfile.php?lid=527
Respiratory Virus detected through Respiratory Viral Panel
- Human coronavirus 229E
- Human coronavirus NL63
- Human coronavirus OC43
- Human coronavirus HKU1
- Human parainfluenza virus 1
- Human parainfluenza virus 2
- Human parainfluenza virus 3
- Human parainfluenza virus 4a
- Influenza A virus (A/Puerto Rico/8/1934(H1N1))
- Influenza A virus (A/ Korea/426/1968(H2N2))
- Influenza A virus (A/New York/392/2004(H3N2))
- Influenza A virus (A/goose/ Guangdong/1/1996(H5N1))
- Influenza A virus (A/Zhejiang/DTIDZJU01/2013(H7N9))
- Influenza A virus (A/Hong Kong/1073/99(H9N2))
- Influenza A virus (A/ Texas/50/2012(H3N2))
- Influenza A virus (A/ Michigan/45/2015(H1N1))
- Influenza B virus (B/Lee/1940)
- Influenza B virus (B/ Wisconsin/01/2010)
- Influenza B virus (B/ Brisbane/60/2008)
- Influenza B virus (B/ Colorado/06/2017)
- Influenza B virus (B/ Washington/02/2019)
- SARS-CoV-2
- Human metapneumovirus (CAN97-83)
- Human adenovirus B1
- Human adenovirus C2
- Human adenovirus E4
- Respiratory syncytial virus (type A)
- Human Respiratory syncytial virus 9320 (type B)
- Human bocavirus 1 (Primate bocaparvovirus 1 isolate st2)
- Human bocavirus 2c PK isolate PK-5510
- Human bocavirus 3
- Human bocavirus 4 NI strain HBoV4- NI-385
- KI polyomavirus Stockholm 60
- WU Polyomavirus
- Human parechovirus type 1 PicoBank/HPeV1/a
- Human parechovirus 6
- Human rhinovirus A89
- Human rhinovirus C (strain 024)
- Human rhinovirus B14
- Human enterovirus C104 strain: AK11
- Human enterovirus C109 isolate NICA08-4327
- Human control genes
Viral Pathogens Panel enables next generation sequencing identification of 66 viral pathogens as listed below
- Adenovirus
- Aichivirus
- Astrovirus
- Chapare virus
- Chikungunya virus
- Coronavirus-229E
- Coronavirus-HKU1
- Coronavirus-OC43
- Coronavirus-NL63
- Coxsackievirus
- Crimean-congo haemorrhagic fever virus
- Dengue virus 1
- Dengue virus 2
- Dengue virus 3
- Dengue virus 4
- Eastern equine encephalitis virus
- Ebola virus
- Enterovirus
- Guanarito virus
- Hantavirus
- Hendra henipavirus
- Hepatitis A virus
- Hepatitis B virus
- Hepatitis C virus
- Hepatitis E virus
- Human Immunodeficiency Virus 1
- Human Immunodeficiency Virus 2
- Influenza A virus
- Influenza B virus
- Japanese encephalitis virus
- Junin virus
- Kyasanur Forest disease virus
- Lassa fever virus
- Lujo hemorrphagic fever virus
- Machupo virus
- Marburg virus
- MERS-CoV
- Metapneumo virus
- Monkeypox virus
- Nipah virus
- Noro virus
- Omsk hemorrhagic fever virus
- Oncolytic human papillomavirus
- Parainfluenza virus
- Parechovirus
- Parvovirus
- Poliovirus
- Polyomavirus
- Respiratory syncytial virus
- Rhinovirus
- Rift Valley fever virus
- Rotavirus
- Rubella virus
- Sabia virus
- Salivirus
- Sapovirus
- SARS-COV
- SARS-COV-2
- Tick-borne encephalitis virus
- Torque Teno virus
- Variola virus
- Venezuelan equine encephalitis virus
- West Nile virus
- Western equine encephalitis virus
- Yellow fever virus
- Zika virus
2.sample requisition form enclosed
3.dr.hemlata@ncdc.gov.in ph : 01123909309, 01123909326
| Section | Tests offered | Section |
| Viral Hepatitis Laboratory | HBsAg (RAPID) | 24 hrs. |
| Anti HCV (RAPID) | 24 hrs. | |
| HBsAg ELISA | 7 days | |
| Anti HCV ELISA | 7 days | |
| Anti HBs ELISA | 7 days | |
| Anti HAV –IgM ELISA | 7 days | |
| Anti HEV-IgM ELISA | 7 days | |
| HBc-IgM ELISA | 7 days | |
| Anti HBc ELISA | 7 days | |
| HBeAg ELISA | 7 days | |
| HBeAb ELISA | 7 days | |
| Anti HAV IgM (CLIA) | 24 -48 hrs | |
| HBsAg (CLIA) | 24 -48 hrs | |
| Anti HCV (CLIA) | 24 -48 hrs | |
| HBc-IgM (CLIA) | 24 -48 hrs | |
| HBV (VIRAL LOAD), COBAS 6800 | 10 days | |
| HCV (VIRAL LOAD), COBAS 6800 | 10 days |
